PUBLICATION

LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation

Authors
Iturbide, A., Pascual-Reguant, L., Fargas, L., Cebrià, J.P., Alsina, B., García de Herreros, A., Peiró, S.
ID
ZDB-PUB-150512-8
Date
2015
Source
Molecular Cell   58(5): 755-66 (Journal)
Registered Authors
Alsina, Berta
Keywords
none
MeSH Terms
  • Amino Acid Oxidoreductases/physiology*
  • Protein Processing, Post-Translational*
  • Animals
  • Zebrafish
  • Oxidation-Reduction
  • Humans
  • TATA-Binding Protein Associated Factors/metabolism*
  • Epigenesis, Genetic
  • Methylation
  • Cell Differentiation*
  • HEK293 Cells
  • Transcription Factor TFIID/metabolism
  • Transcription Factor TFIID/physiology*
  • Neural Stem Cells/physiology*
(all 14)
PubMed
25959397 Full text @ Mol. Cell
Abstract
Protein function is often regulated and controlled by posttranslational modifications, such as oxidation. Although oxidation has been mainly considered to be uncontrolled and nonenzymatic, many enzymatic oxidations occur on enzyme-selected lysine residues; for instance, LOXL2 oxidizes lysines by converting the ε-amino groups into aldehyde groups. Using an unbiased proteomic approach, we have identified methylated TAF10, a member of the TFIID complex, as a LOXL2 substrate. LOXL2 oxidation of TAF10 induces its release from its promoters, leading to a block in TFIID-dependent gene transcription. In embryonic stem cells, this results in the inactivation of the pluripotency genes and loss of the pluripotent capacity. During zebrafish development, the absence of LOXL2 resulted in the aberrant overexpression of the neural progenitor gene Sox2 and impaired neural differentiation. Thus, lysine oxidation of the transcription factor TAF10 is a controlled protein modification and demonstrates a role for protein oxidation in regulating pluripotency genes.
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Sequence Targeting Reagents
Target Reagent Reagent Type
loxl2aMO3-loxl2aMRPHLNO
loxl2bMO1-loxl2bMRPHLNO
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Fish
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