PUBLICATION

Melatonin Is Required for the Circadian Regulation of Sleep

Authors
Gandhi, A.V., Mosser, E.A., Oikonomou, G., Prober, D.A.
ID
ZDB-PUB-150311-4
Date
2015
Source
Neuron   85(6): 1193-9 (Journal)
Registered Authors
Prober, David
Keywords
none
MeSH Terms
  • Circadian Clocks/genetics
  • Sleep/physiology*
  • Animals
  • Animals, Genetically Modified/genetics
  • Circadian Rhythm/genetics
  • Circadian Rhythm/physiology*
  • Light
  • Melatonin/metabolism*
  • Genotype
  • Zebrafish/physiology*
  • Arylalkylamine N-Acetyltransferase/genetics
(all 11)
PubMed
25754820 Full text @ Neuron
Abstract
Sleep is an evolutionarily conserved behavioral state whose regulation is poorly understood. A classical model posits that sleep is regulated by homeostatic and circadian mechanisms. Several factors have been implicated in mediating the homeostatic regulation of sleep, but molecules underlying the circadian mechanism are unknown. Here we use animals lacking melatonin due to mutation of arylalkylamine N-acetyltransferase 2 (aanat2) to show that melatonin is required for circadian regulation of sleep in zebrafish. Sleep is dramatically reduced at night in aanat2 mutants maintained in light/dark conditions, and the circadian regulation of sleep is abolished in free-running conditions. We find that melatonin promotes sleep downstream of the circadian clock as it is not required to initiate or maintain circadian rhythms. Additionally, we provide evidence that melatonin may induce sleep in part by promoting adenosine signaling, thus potentially linking circadian and homeostatic control of sleep.
Genes / Markers
Marker Marker Type Name
aanat1GENEarylalkylamine N-acetyltransferase 1
aanat2GENEarylalkylamine N-acetyltransferase 2
per1bGENEperiod circadian clock 1b
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Figures
Figure Gallery (1 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ct801
    Small Deletion
    ct822TgTransgenic Insertion
      ct823
        Small Deletion
        g1TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          aanat1CRISPR1-aanat1CRISPR
          1 - 1 of 1
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          Fish
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-serotoninpolyclonal
            Rabbit
            1 - 1 of 1
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            CFPEFGCFP
            LuciferaseEFGLuciferase
            NTREFGNTR
            1 - 3 of 3
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            Mapping
            No data available