PUBLICATION
Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs
- Authors
- Rodríguez-Fraticelli, A.E., Bagwell, J., Bosch-Fortea, M., Boncompain, G., Reglero-Real, N., García-León, M.J., Andrés, G., Toribio, M.L., Alonso, M.A., Millán, J., Perez, F., Bagnat, M., Martín-Belmonte, F.
- ID
- ZDB-PUB-150224-2
- Date
- 2015
- Source
- Nature cell biology 17(3): 241-50 (Journal)
- Registered Authors
- Bagnat, Michel
- Keywords
- none
- MeSH Terms
-
- Endosomes/metabolism*
- Endosomes/ultrastructure
- Zebrafish
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Signal Transduction
- Cell Proliferation
- Cell Differentiation
- Gene Expression Regulation, Developmental*
- Kidney Tubules/metabolism
- Kidney Tubules/ultrastructure
- Cell Polarity
- Embryo, Nonmammalian
- Cell Line
- Epithelial Cells/metabolism*
- Epithelial Cells/ultrastructure
- Myelin and Lymphocyte-Associated Proteolipid Proteins/genetics
- Myelin and Lymphocyte-Associated Proteolipid Proteins/metabolism
- Endocytosis
- Adaptor Proteins, Vesicular Transport/genetics
- Adaptor Proteins, Vesicular Transport/metabolism
- SNARE Proteins/genetics
- SNARE Proteins/metabolism
- Lysosomes/metabolism*
- Lysosomes/ultrastructure
- Mice
- Epithelium/metabolism*
- Epithelium/ultrastructure
- Animals
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- PubMed
- 25706235 Full text @ Nat. Cell Biol.
Citation
Rodríguez-Fraticelli, A.E., Bagwell, J., Bosch-Fortea, M., Boncompain, G., Reglero-Real, N., García-León, M.J., Andrés, G., Toribio, M.L., Alonso, M.A., Millán, J., Perez, F., Bagnat, M., Martín-Belmonte, F. (2015) Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs. Nature cell biology. 17(3):241-50.
Abstract
Epithelial organs develop through tightly coordinated events of cell proliferation and differentiation in which endocytosis plays a major role. Despite recent advances, how endocytosis regulates the development of vertebrate organs is still unknown. Here we describe a mechanism that facilitates the apical availability of endosomal SNARE receptors for epithelial morphogenesis through the developmental upregulation of plasmolipin (pllp) in a highly endocytic segment of the zebrafish posterior midgut. The protein PLLP (Pllp in fish) recruits the clathrin adaptor EpsinR to sort the SNARE machinery of the endolysosomal pathway into the subapical compartment, which is a switch for polarized endocytosis. Furthermore, PLLP expression induces apical Crumbs internalization and the activation of the Notch signalling pathway, both crucial steps in the acquisition of cell polarity and differentiation of epithelial cells. We thus postulate that differential apical endosomal SNARE sorting is a mechanism that regulates epithelial patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping