PUBLICATION
β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member
- Authors
- Reuter, H., März, M., Vogg, M.C., Eccles, D., Grífol-Boldú, L., Wehner, D., Owlarn, S., Adell, T., Weidinger, G., Bartscherer, K.
- ID
- ZDB-PUB-150106-1
- Date
- 2015
- Source
- Cell Reports 10(2): 253-65 (Journal)
- Registered Authors
- Wehner, Daniel, Weidinger, Gilbert
- Keywords
- none
- MeSH Terms
-
- Repressor Proteins/antagonists & inhibitors
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Stem Cells/cytology
- Stem Cells/metabolism
- Cytoskeletal Proteins/antagonists & inhibitors
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism
- Transcriptome
- Collagen/metabolism
- Zebrafish
- RNA Interference
- Regeneration
- Helminth Proteins/antagonists & inhibitors
- Helminth Proteins/genetics
- Helminth Proteins/metabolism*
- RNA, Double-Stranded/metabolism
- beta Catenin/antagonists & inhibitors
- beta Catenin/genetics
- beta Catenin/metabolism*
- Animals
- Body Patterning/physiology*
- Base Sequence
- Molecular Sequence Data
- Wnt Proteins/antagonists & inhibitors
- Wnt Proteins/metabolism
- Wnt Signaling Pathway
- Planarians
- PubMed
- 25558068 Full text @ Cell Rep.
Citation
Reuter, H., März, M., Vogg, M.C., Eccles, D., Grífol-Boldú, L., Wehner, D., Owlarn, S., Adell, T., Weidinger, G., Bartscherer, K. (2015) β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member. Cell Reports. 10(2):253-65.
Abstract
Wnt/β-catenin signaling regulates tissue homeostasis and regeneration in metazoans. In planarians-flatworms with high regenerative potential-Wnt ligands are thought to control tissue polarity by shaping a β-catenin activity gradient along the anterior-posterior axis, yet the downstream mechanisms are poorly understood. We performed an RNA sequencing (RNA-seq)-based screen and identified hundreds of β-catenin-dependent transcripts, of which several were expressed in muscle tissue and stem cells in a graded fashion. In particular, a teashirt (tsh) ortholog was induced in a β-catenin-dependent manner during regeneration in planarians and zebrafish, and RNAi resulted in two-headed planarians. Strikingly, intact planarians depleted of tsh induced anterior markers and slowly transformed their tail into a head, reminiscent of β-catenin RNAi phenotypes. Given that β-catenin RNAi enhanced the formation of muscle cells expressing anterior determinants in tail regions, our study suggests that this pathway controls tissue polarity through regulating the identity of differentiating cells during homeostasis and regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping