PUBLICATION
Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish
- Authors
- Zou, P.F., Chang, M.X., Li, Y., Zhang, S.H., Fu, J.P., Chen, S.N., Nie, P.
- ID
- ZDB-PUB-141220-4
- Date
- 2015
- Source
- Fish & shellfish immunology 43(1): 13-24 (Journal)
- Registered Authors
- Chang, Mingxian, Li, Ying, Nie, Pin
- Keywords
- RIG-Ib/MAVS-mediated signaling pathway, mitochondrial antiviral signaling protein, retinoic acid-inducible gene-I, type I IFN production, variant
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antiviral Agents/metabolism
- Cell Line
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Edwardsiella tarda/physiology
- Enterobacteriaceae Infections/genetics
- Enterobacteriaceae Infections/metabolism
- Enterobacteriaceae Infections/veterinary*
- Enterobacteriaceae Infections/virology
- Fish Diseases/genetics*
- Fish Diseases/metabolism
- Fish Diseases/virology
- Gene Expression Regulation*
- Interferon Type I/genetics
- Interferon Type I/metabolism
- Molecular Sequence Data
- Promoter Regions, Genetic
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Rhabdoviridae/physiology
- Rhabdoviridae Infections/genetics
- Rhabdoviridae Infections/metabolism
- Rhabdoviridae Infections/veterinary*
- Rhabdoviridae Infections/virology
- Sequence Alignment/veterinary
- Signal Transduction*
- Zebrafish*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 25524497 Full text @ Fish Shellfish Immunol.
Citation
Zou, P.F., Chang, M.X., Li, Y., Zhang, S.H., Fu, J.P., Chen, S.N., Nie, P. (2015) Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish. Fish & shellfish immunology. 43(1):13-24.
Abstract
As an intracellular pattern recognition receptor (PRR), the retinoic acid-inducible gene-I (RIG-I) is responsible for the recognition of cytosolic viral nucleic acids and the production of type I interferons (IFNs). In the present study, an insertion variant of RIG-I with 38 amino acids inserted in the N-terminal CARD2 domain, as well as the typical type, named as RIG-Ia and RIG-Ib respectively were identified in zebrafish. RIG-Ia and RIG-Ib were all up-regulated following the infection of a negative ssRNA virus, the Spring Viremia of Carp Virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, indicating the RLR may have a role in the recognition of both viruses and bacteria. The over-expression of RIG-Ib in cultured fish cells resulted in significant increase in type I IFN promoter activity, and in protection against SVCV infection, whereas the over-expression of RIG-Ia had no direct effect on IFN activation nor antiviral response. Furthermore, it was revealed that both RIG-Ia and RIG-Ib were associated with the downstream molecular mitochondrial antiviral signaling protein, MAVS, and interestingly RIG-Ia when co-transfected with RIG-Ib or MAVS, induced a significantly higher level of type I IFN promoter activity and the expression level of Mx and IRF7, implying that the RIG-Ia may function as an enhancer in the RIG-Ib/MAVS-mediated signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping