PUBLICATION

High-frequency dual mode pulsed wave Doppler imaging for monitoring the functional regeneration of adult zebrafish hearts

Authors
Kang, B.J., Park, J., Kim, J., Kim, H.H., Lee, C., Hwang, J.Y., Lien, C.L., Shung, K.K.
ID
ZDB-PUB-141217-10
Date
2015
Source
Journal of the Royal Society, Interface   12(103): 2968-74 (Journal)
Registered Authors
Lien, Ching-Ling (Ellen)
Keywords
Doppler flow, echocardiography, heart regeneration, tissue Doppler, zebrafish
MeSH Terms
  • Animals
  • Diastole*
  • Echocardiography, Doppler*
  • Heart/physiology*
  • Regeneration*
  • Zebrafish*
PubMed
25505135 Full text @ J. R. Soc. Interface
Abstract
Adult zebrafish is a well-known small animal model for studying heart regeneration. Although the regeneration of scars made by resecting the ventricular apex has been visualized with histological methods, there is no adequate imaging tool for tracking the functional recovery of the damaged heart. For this reason, high-frequency Doppler echocardiography using dual mode pulsed wave Doppler, which provides both tissue Doppler (TD) and Doppler flow in a same cardiac cycle, is developed with a 30 MHz high-frequency array ultrasound imaging system. Phantom studies show that the Doppler flow mode of the dual mode is capable of measuring the flow velocity from 0.1 to 15 cm s(-1) with high accuracy (p-value = 0.974 > 0.05). In the in vivo study of zebrafish, both TD and Doppler flow signals were simultaneously obtained from the zebrafish heart for the first time, and the synchronized valve motions with the blood flow signals were identified. In the longitudinal study on the zebrafish heart regeneration, the parameters for diagnosing the diastolic dysfunction, for example, E/Em < 10, E/A < 0.14 for wild-type zebrafish, were measured, and the type of diastolic dysfunction caused by the amputation was found to be similar to the restrictive filling. The diastolic function was fully recovered within four weeks post-amputation.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping