PUBLICATION
Zebrafish embryo intersegmental vessels: a tool for investigating sprouting angiogenesis
- Authors
- Tobia, C., Gariano, G., Guerra, J., Presta, M.
- ID
- ZDB-PUB-141204-7
- Date
- 2015
- Source
- Methods in molecular biology (Clifton, N.J.) 1214: 173-84 (Chapter)
- Registered Authors
- Presta, Marco, Tobia, Chiara
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blood Vessels/embryology*
- Blood Vessels/physiology*
- Embryo, Nonmammalian/blood supply*
- In Situ Hybridization/methods*
- Neovascularization, Physiologic*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 25468604 Full text @ Meth. Mol. Biol.
Citation
Tobia, C., Gariano, G., Guerra, J., Presta, M. (2015) Zebrafish embryo intersegmental vessels: a tool for investigating sprouting angiogenesis. Methods in molecular biology (Clifton, N.J.). 1214:173-84.
Abstract
Angiogenesis leads to the formation of the intersegmental vessels (ISVs) of the trunk in teleost zebrafish (Danio rerio) embryo. Here we describe experimental procedures, including in vivo observation of transgenic zebrafish embryo and whole mount in situ hybridization, to investigate ISV development in zebrafish embryos and assess the effect of antiangiogenic compounds on these vessels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping