PUBLICATION
Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish
- Authors
- Crucke, J., Huysseune, A.
- ID
- ZDB-PUB-141114-5
- Date
- 2015
- Source
- Journal of dental research 94(1): 157-65 (Journal)
- Registered Authors
- Huysseune, Ann
- Keywords
- Danio rerio, SU5416, blood supply, dentition, tooth replacement, vascular endothelial growth factor
- MeSH Terms
-
- Alkaline Phosphatase/analysis
- Angiogenesis Inhibitors/pharmacology
- Animals
- Blood Vessels/drug effects
- Cell Differentiation/drug effects
- Embryo, Nonmammalian/drug effects
- Indoles/pharmacology
- Larva/drug effects
- Neovascularization, Physiologic/drug effects
- Odontogenesis/drug effects*
- Pyrroles/pharmacology
- Tooth/blood supply
- Tooth/drug effects
- Tooth/growth & development*
- Tooth Germ/drug effects
- Vascular Endothelial Growth Factor A/antagonists & inhibitors*
- Zebrafish/growth & development*
- Zebrafish Proteins/antagonists & inhibitors*
- PubMed
- 25391620 Full text @ J. Dent. Res.
Citation
Crucke, J., Huysseune, A. (2015) Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish. Journal of dental research. 94(1):157-65.
Abstract
The dentition in zebrafish is extremely and richly vascularized, but the function of the vasculature, in view of the continuous replacement of the teeth, remains elusive. Through application of SU5416, a vascular endothelial growth factor receptor inhibitor, we studied the role of the blood vessels in the dentition of the zebrafish. We were unable to show an effect on the development of first-generation teeth as well as first tooth replacement. However, in juvenile fish, a delay was observed in the developmental state of the replacement tooth compared with what was expected based on the maturation state of the functional tooth. Furthermore, we observed a difference between treated and nontreated fish in the distance between blood vessels and developing replacement teeth. In conclusion, our results provide support for a nutritive, rather than an inductive, function of the vasculature in the process of tooth development and replacement.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping