PUBLICATION

Myosin Vb Mediated Plasma Membrane Homeostasis Regulates Peridermal Cell Size and Maintains Tissue Homeostasis in the Zebrafish Epidermis

Authors
Sonal, ., Sidhaye, J., Phatak, M., Banerjee, S., Mulay, A., Deshpande, O., Bhide, S., Jacob, T., Gehring, I., Nuesslein-Volhard, C., Sonawane, M.
ID
ZDB-PUB-140919-1
Date
2014
Source
PLoS Genetics   10: e1004614 (Journal)
Registered Authors
Gehring, Ines, Jacob, Tressa Panikulangara, Nüsslein-Volhard, Christiane, Sidhaye, Jaydeep, Sonawane, Mahendra
Keywords
Myosins, Embryos, Cell membranes, Epidermis, Endocytosis, Vesicles, Larvae, Dextran
MeSH Terms
  • Animals
  • Cell Count
  • Cell Membrane/metabolism*
  • Cell Size
  • Embryo, Nonmammalian
  • Endocytosis
  • Endosomes/metabolism
  • Epidermis/cytology*
  • Epidermis/embryology
  • Epidermis/metabolism*
  • Genetic Loci
  • Homeostasis*
  • Lysosomes/metabolism
  • Models, Biological
  • Mutation
  • Myosin Type V/deficiency
  • Myosin Type V/genetics
  • Myosin Type V/metabolism*
  • Phenotype
  • Zebrafish
PubMed
25233349 Full text @ PLoS Genet.
Abstract
The epidermis is a stratified epithelium, which forms a barrier to maintain the internal milieu in metazoans. Being the outermost tissue, growth of the epidermis has to be strictly coordinated with the growth of the embryo. The key parameters that determine tissue growth are cell number and cell size. So far, it has remained unclear how the size of epidermal cells is maintained and whether it contributes towards epidermal homeostasis. We have used genetic analysis in combination with cellular imaging to show that zebrafish goosepimples/myosin Vb regulates plasma membrane homeostasis and is involved in maintenance of cell size in the periderm, the outermost epidermal layer. The decrease in peridermal cell size in Myosin Vb deficient embryos is compensated by an increase in cell number whereas decrease in cell number results in the expansion of peridermal cells, which requires myosin Vb (myoVb) function. Inhibition of cell proliferation as well as cell size expansion results in increased lethality in larval stages suggesting that this two-way compensatory mechanism is essential for growing larvae. Our analyses unravel the importance of Myosin Vb dependent cell size regulation in epidermal homeostasis and demonstrate that the epidermis has the ability to maintain a dynamic balance between cell size and cell number.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes