PUBLICATION
Zebrafish xenotransplantation model for cancer stem-like cell study and high-throughput screening of inhibitors
- Authors
- Zhang, B., Shimada, Y., Kuroyanagi, J., Nishimura, Y., Umemoto, N., Nomoto, T., Shintou, T., Miyazaki, T., Tanaka, T.
- ID
- ZDB-PUB-140912-2
- Date
- 2014
- Source
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 35(12): 11861-9 (Journal)
- Registered Authors
- Tanaka, Toshio
- Keywords
- none
- MeSH Terms
-
- Animals
- Antineoplastic Agents/pharmacology*
- Cell Line, Tumor
- Disease Models, Animal
- Drug Screening Assays, Antitumor*
- Female
- Hep G2 Cells
- High-Throughput Screening Assays*
- Humans
- K562 Cells
- Male
- Neoplastic Stem Cells/drug effects*
- Neoplastic Stem Cells/metabolism*
- Neoplastic Stem Cells/transplantation
- Transplantation, Heterologous
- Zebrafish
- PubMed
- 25209178 Full text @ Tumour Biol.
Citation
Zhang, B., Shimada, Y., Kuroyanagi, J., Nishimura, Y., Umemoto, N., Nomoto, T., Shintou, T., Miyazaki, T., Tanaka, T. (2014) Zebrafish xenotransplantation model for cancer stem-like cell study and high-throughput screening of inhibitors. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(12):11861-9.
Abstract
Xenotransplantation studies are important tools for studying cancer biology, especially for assaying tumor cell malignancy and providing cancer information in vivo. Cancer stem-like cells (CSCs) have been identified in many cancer types to drive tumor growth and recurrence, from "keeping" to "keep" resistant to chemotherapy and radiation therapy. In this study, we developed the xenotransplantation of CSCs derived from the leukemia and solid tumor cell lines using the zebrafish models. In adult zebrafish, we investigated that the xenografted leukemia stem cells (LSCs) from K562 cells could proliferate in vivo and keep the cancer property by re-transplantation. As for the solid tumor, these CSCs from DU145 cells (human prostate cancer) and HepG2 cells (human liver cancer) could form the tumor mass and even metastasis after xenotransplantation. In addition, the zebrafish embryos with CSC xenotransplantation could evaluate docetaxel in vivo efficiently and be available to screen the novel inhibitors by high-throughput manner. In summary, these zebrafish xenotransplantation models devote a good platform for the CSC mechanism investigation and anti-CSC inhibitor screening.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping