PUBLICATION
Prochloraz causes irreversible masculinization of zebrafish (Danio rerio)
- Authors
- Baumann, L., Knörr, S., Keiter, S., Nagel, T., Segner, H., Braunbeck, T.
- ID
- ZDB-PUB-140829-2
- Date
- 2015
- Source
- Environmental science and pollution research international 22(21): 16417-22 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- Zebrafish, Gonad histology, Sexual differentiation, Endocrine disruptor, Aromatase inhibitor, Fungicide
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Endocrine Disruptors/toxicity*
- Female
- Imidazoles/toxicity*
- Male
- Sex Differentiation/drug effects
- Sex Ratio
- Sexual Development/drug effects*
- Vitellogenins/metabolism
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish/physiology
- PubMed
- 25163568 Full text @ Environ. Sci. Pollut. Res. Int.
Citation
Baumann, L., Knörr, S., Keiter, S., Nagel, T., Segner, H., Braunbeck, T. (2015) Prochloraz causes irreversible masculinization of zebrafish (Danio rerio). Environmental science and pollution research international. 22(21):16417-22.
Abstract
The aim of the present study was to investigate the persistence of endocrine effects by prochloraz, a fungicide known to have multiple effects on the endocrine system of vertebrates. Since discontinuous exposure is particularly relevant in aquatic ecosystems, an exposure scenario with an exposure phase and a subsequent recovery period was chosen to assess the potential for reversibility of effects by prochloraz on the sexual development of zebrafish (Danio rerio). Zebrafish were exposed to different concentrations of prochloraz (10-300 μg/L) until 60 days post hatch (dph), which includes the period of sexual differentiation. For the subsequent 40 days, fish were either held in clean water for depuration or under further continuous exposure. Histological investigations of the gonads revealed persistent effects on sexual differentiation. The sex ratio was skewed towards males and significantly more intersex individuals were found after exposure to prochloraz at 60 dph. No intersex fish, but masculinized sex ratios were still present after the depuration period, documenting that prochloraz irreversibly affects the sexual development of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping