PUBLICATION

Functional analysis of hox genes in zebrafish

Authors
Ladam, F., Sagerström, C.G.
ID
ZDB-PUB-140826-6
Date
2014
Source
Methods in molecular biology (Clifton, N.J.)   1196: 133-44 (Chapter)
Registered Authors
Sagerström, Charles
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Embryo, Nonmammalian/metabolism
  • Female
  • Gene Expression
  • Gene Targeting
  • Genetic Vectors
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Male
  • Transgenes
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
PubMed
25151162 Full text @ Meth. Mol. Biol.
Abstract
The zebrafish model organism is well suited to study the role of specific genes, such as hox genes, in embryogenesis and organ function. The ability to modulate the activity of hox genes in living zebrafish embryos represents a cornerstone of such functional analyses. In this chapter we outline the basic methodology for nucleic acid injections into 1-2-cell-stage zebrafish embryos. We also report variations in this method to allow injection of mRNA, DNA, and antisense oligonucleotides to either overexpress, knock down, or knock out specific genes in zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes