Functional analysis of hox genes in zebrafish
- Ladam, F., Sagerström, C.G.
- Methods in molecular biology (Clifton, N.J.) 1196: 133-44 (Chapter)
- Registered Authors
- Sagerström, Charles
- MeSH Terms
- Animals, Genetically Modified
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryo, Nonmammalian/metabolism
- Gene Expression
- Gene Targeting
- Genetic Vectors
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism*
- 25151162 Full text @ Meth. Mol. Biol.
Ladam, F., Sagerström, C.G. (2014) Functional analysis of hox genes in zebrafish. Methods in molecular biology (Clifton, N.J.). 1196:133-44.
The zebrafish model organism is well suited to study the role of specific genes, such as hox genes, in embryogenesis and organ function. The ability to modulate the activity of hox genes in living zebrafish embryos represents a cornerstone of such functional analyses. In this chapter we outline the basic methodology for nucleic acid injections into 1-2-cell-stage zebrafish embryos. We also report variations in this method to allow injection of mRNA, DNA, and antisense oligonucleotides to either overexpress, knock down, or knock out specific genes in zebrafish embryos.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes