PUBLICATION

Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development

Authors
Schupp, M.O., Waas, M., Chun, C.Z., Ramchandran, R.
ID
ZDB-PUB-140702-1
Date
2014
Source
Developmental Biology   393(1): 71-83 (Journal)
Registered Authors
Chun, Chang Zoon, Ramchandran, Ramani, Schupp, Marcus
Keywords
Zebrafish, Transcription, Cardiac disc, Etv2, Scl
MeSH Terms
  • Cell Line
  • Transcription Factors/genetics
  • Cell Differentiation/genetics
  • Promoter Regions, Genetic
  • Heart/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Gene Silencing
  • Animals
  • Erythrocytes/cytology
  • Animals, Genetically Modified
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
  • Transcription, Genetic/genetics*
  • Gene Knockdown Techniques
  • Endothelial Cells/cytology
  • Morpholinos/genetics
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/embryology*
  • Transgenes
  • Proto-Oncogene Proteins/genetics
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Embryonic Stem Cells
  • Cell Lineage
  • Gene Expression Regulation, Developmental
(all 25)
PubMed
24984259 Full text @ Dev. Biol.
Abstract
E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mw201TgTransgenic Insertion
    mw202TgTransgenic Insertion
      mw203TgTransgenic Insertion
        mw204TgTransgenic Insertion
          mw205TgTransgenic Insertion
            mw206TgTransgenic Insertion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              etsrpMO2-etsrpMRPHLNO
              gata5MO5-gata5MRPHLNO
              hand2MO2-hand2MRPHLNO
              nkx2.5MO3-nkx2.5MRPHLNO
              tal1MO4-tal1MRPHLNO
              tp53MO4-tp53MRPHLNO
              1 - 6 of 6
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              Fish
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              KaedeEFGKaede
              mCherryEFGmCherry
              1 - 3 of 3
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              Mapping
              No data available