PUBLICATION
The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs
- Authors
- Chiavacci, E., Rizzo, M., Pitto, L., Patella, F., Evangelista, M., Mariani, L., Rainaldi, G.
- ID
- ZDB-PUB-140621-11
- Date
- 2015
- Source
- Cytotechnology 67(6): 969-75 (Journal)
- Registered Authors
- Chiavacci, Elena
- Keywords
- microRNAs, Tumor angiogenesis, Zebrafish/tumor xenografts, Prostate tumor cells
- MeSH Terms
- none
- PubMed
- 24947063 Full text @ Cytotechnology
Citation
Chiavacci, E., Rizzo, M., Pitto, L., Patella, F., Evangelista, M., Mariani, L., Rainaldi, G. (2015) The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs. Cytotechnology. 67(6):969-75.
Abstract
The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. After transfection, DU-145 cells were injected close to the developing subintestinal vessels of transgenic Tg(Kdrl:eGFP)s843 zebrafish embryos that express green fluorescent protein under the control of Kdrl promoter. At 72 h post-fertilization, we observed that green fluorescent protein-positive neo-vessels infiltrated the graft of DU-145 transfected with miR-125a, miR-320, and miR-487b. Vice versa, neo-vessel formation and tumor cell infiltration were inhibited when DU-145 cells transfected with miR-492 were used. These results indicated that the zebrafish/tumor xenograft assay was adequate to identify microRNAs able to suppress the release of angiogenic growth factors by angiogenic tumor cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping