PUBLICATION

On the Crucial Cerebellar Wound Healing-Related Pathways and Their Cross-Talks after Traumatic Brain Injury in Danio rerio

Authors
Wu, C.C., Tsai, T.H., Chang, C., Lee, T.T., Lin, C., Cheng, I.H., Sun, M.C., Chuang, Y.J., Chen, B.S.
ID
ZDB-PUB-140614-3
Date
2014
Source
PLoS One   9: e97902 (Journal)
Registered Authors
Chuang, Yung-Jen
Keywords
none
Datasets
GEO:GSE56375
MeSH Terms
  • Protein Array Analysis
  • Phosphatidylinositol 3-Kinase/metabolism
  • Disease Models, Animal
  • Animals
  • Zebrafish
  • Protein Interaction Maps
  • Models, Neurological
  • Signal Transduction
  • Cerebellum/metabolism
  • Cerebellum/physiopathology*
  • Humans
  • Brain Injuries/metabolism*
  • Brain Injuries/physiopathology
  • Wound Healing*
  • Proteomics/methods*
  • Zebrafish Proteins/metabolism*
  • Neurogenesis
(all 17)
PubMed
24926785 Full text @ PLoS One
Abstract

Upon injury, the direct damage and the subsequent secondary injury in the brain often result in chronic neurological disorders. Due to multifactorial nature of secondary injury and subsequent complex cellular responses, much of the underlying mechanisms are unclear. This study used an adult zebrafish cerebellum injury model to investigate the phenotypes and the secondary injury responses for recovery mechanisms of injured brain. Using the time course microarray analysis, a candidate protein-protein interaction (PPI) network was refined as cerebellar wound healing PPI network by dynamic modeling and big data mining. Pathway enrichment and ontological analysis were incorporated into the refined network to highlight the main molecular scheme of cerebellar wound healing. Several significant pathways, including chemokine, Phosphatidylinositide 3-kinases, and axon guidance signaling pathway and their cross-talks through PI3K, PAK2, and PLXNA3 were identified to coordinate for neurogenesis and angiogenesis, which are essential for the restoration of the injured brain. Our finding provides an insight into the molecular restoration mechanisms after traumatic brain injury, and open up new opportunity to devise the treatment for traumatic brain injury in human.

Genes / Markers
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Allele Construct Type Affected Genomic Region
zf567TgTransgenic Insertion
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    Marker Marker Type Name
    EGFPEFGEGFP
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    Citation
    Wu, C.C., Tsai, T.H., Chang, C., Lee, T.T., Lin, C., Cheng, I.H., Sun, M.C., Chuang, Y.J., Chen, B.S. (2014) On the Crucial Cerebellar Wound Healing-Related Pathways and Their Cross-Talks after Traumatic Brain Injury in Danio rerio. PLoS One. 9:e97902.