ZFIN ID: ZDB-PUB-140606-2
Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications
Zhou, Y., Cattley, R.T., Cario, C.L., Bai, Q., Burton, E.A.
Date: 2014
Source: Nature Protocols   9: 1533-1548 (Journal)
Registered Authors: Burton, Edward A.
Keywords: none
MeSH Terms:
  • Animals
  • Image Processing, Computer-Assisted/instrumentation
  • Image Processing, Computer-Assisted/methods*
  • Larva
  • Motor Activity/physiology*
  • Software*
  • Video Recording/instrumentation
  • Video Recording/methods
  • Zebrafish/physiology*
PubMed: 24901738 Full text @ Nat. Protoc.
This article describes a method to quantify the movements of larval zebrafish in multiwell plates, using the open-source MATLAB applications LSRtrack and LSRanalyze. The protocol comprises four stages: generation of high-quality, flatly illuminated video recordings with exposure settings that facilitate object recognition; analysis of the resulting recordings using tools provided in LSRtrack to optimize tracking accuracy and motion detection; analysis of tracking data using LSRanalyze or custom MATLAB scripts; and implementation of validation controls. The method is reliable, automated and flexible, requires <1 h of hands-on work for completion once optimized and shows excellent signal:noise characteristics. The resulting data can be analyzed to determine the following: positional preference; displacement, velocity and acceleration; and duration and frequency of movement events and rest periods. This approach is widely applicable to the analysis of spontaneous or stimulus-evoked zebrafish larval neurobehavioral phenotypes resulting from a broad array of genetic and environmental manipulations, in a multiwell plate format suitable for high-throughput applications.