PUBLICATION
The influence of probiotics on zebrafish danio rerio innate immunity and hepatic stress
- Authors
- Gioacchini, G., Giorgini, E., Olivotto, I., Maradonna, F., Merrifield, D.L., Carnevali, O.
- ID
- ZDB-PUB-140513-479
- Date
- 2014
- Source
- Zebrafish 11: 98-106 (Journal)
- Registered Authors
- Carnevali, Oliana
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/immunology
- Biomarkers/metabolism
- Female
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Immunity, Innate*
- Lacticaseibacillus rhamnosus/chemistry
- Lacticaseibacillus rhamnosus/physiology*
- Liver/immunology*
- Oxidative Stress
- Probiotics/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Stress, Physiological
- Zebrafish/genetics
- Zebrafish/immunology*
- Zebrafish/microbiology*
- PubMed
- 24564619 Full text @ Zebrafish
Citation
Gioacchini, G., Giorgini, E., Olivotto, I., Maradonna, F., Merrifield, D.L., Carnevali, O. (2014) The influence of probiotics on zebrafish danio rerio innate immunity and hepatic stress. Zebrafish. 11:98-106.
Abstract
Abstract In this study, the effects of probiotic administration on zebrafish Danio rerio intestinal innate immunity and hepatic stress were evaluated. Zebrafish adults were treated for 10 days with the probiotic Lactobacillus rhamnosus IMC 501(®). To assess the effects at the molecular level, the mRNA levels of genes involved in the innate immune system, stress response, oxidative stress, and apoptosis were quantified by real-time polymerase chain reaction. An increase of biomarkers related to innate immune responses was observed in intestinal tissue from the probiotic-treated fish compared with the control fish. In addition, a decrease in the abundance of stress and apoptotic-related genes was observed in the liver of the probiotic-fed fish. Finally, imaging Fourier transform infrared analysis was conducted on liver sections and the data obtained confirmed that probiotic administration decreased oxidative stress levels, decreased DNA damage, and increased lipid saturation levels. Overall, the results show that probiotic administration may enhance zebrafish welfare by modulating the innate immune response and improving hepatic stress tolerance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping