ZFIN ID: ZDB-PUB-140218-1
Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic
Gioacchini, G., Valle, L.D., Benato, F., Fimia, G.M., Nardacci, R., Ciccosanti, F., Piacentini, M., Borini, A., and Carnevali, O.
Date: 2013
Source: Reproduction, fertility, and development   25(8): 1115-1125 (Journal)
Registered Authors: Benato, Francesca, Carnevali, Oliana, Piacentini, Mauro
Keywords: none
MeSH Terms:
  • Animals
  • Apoptosis*
  • Apoptosis Regulatory Proteins/antagonists & inhibitors
  • Apoptosis Regulatory Proteins/genetics
  • Apoptosis Regulatory Proteins/metabolism
  • Autophagy*
  • Diet/veterinary*
  • Female
  • Gene Expression Regulation, Developmental
  • Insulin-Like Growth Factor I
  • Insulin-Like Growth Factor II/biosynthesis
  • Insulin-Like Growth Factor II/genetics
  • Insulin-Like Growth Factor II/metabolism
  • Lactobacillus rhamnosus/growth & development*
  • Microscopy, Electron, Transmission
  • Microtubule-Associated Proteins/biosynthesis
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/metabolism
  • Oogenesis
  • Ovarian Follicle/cytology*
  • Ovarian Follicle/metabolism
  • Ovarian Follicle/ultrastructure
  • Probiotics*
  • Proto-Oncogene Proteins c-myc/antagonists & inhibitors
  • Proto-Oncogene Proteins c-myc/genetics
  • Proto-Oncogene Proteins c-myc/metabolism
  • RNA, Messenger/metabolism
  • Somatomedins/biosynthesis
  • Somatomedins/genetics
  • Somatomedins/metabolism
  • Vitellogenesis
  • Zebrafish/growth & development*
  • Zebrafish/microbiology
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 23195281 Full text @ Reprod. Fertil. Dev.

The present study investigated autophagic processes in Danio rerio preovulatory follicles (Stage III and IV). There were more autophagosomes, as revealed by electron microscopy, in follicles from females fed the probiotic Lactobacillus rhamnosus IMC 501. This was confirmed by increased expression of genes involved in the autophagic process, namely ambra1, becn1, lc3 and uvrag. In addition, preovulatory follicles from females fed the probiotic contained more microtubule-associated protein 1 light chain 3 isoform II (LC3-II) and less p62 protein. The increased autophagy in preovulatory follicles from females fed the probiotic was concomitant with a decrease in the apoptotic process in the ovary, as evidenced by terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling analysis and confirmed by lower expression of genes involved in apoptosis (i.e. p53, bax, apaf and cas3) and higher expression as igfII and igf1r. The results of the present study provide preliminary evidence of the involvement of autophagy during follicle development in the zebrafish ovary. In addition, we have demonstrated for the first time that a functional food, such as L. rhamnosus IMC 501, can modulate the balance between apoptosis and autophagy that regulates ovary physiology in zebrafish by inhibiting follicular apoptosis and improving follicular survival.