ZFIN ID: ZDB-PUB-131203-32
Transforming growth factor beta signalling controls human breast cancer metastasis in a zebrafish xenograft model
Drabsch, Y., He, S., Zhang, L., Snaar-Jagalska, B.E., and Ten Dijke, P.
Date: 2013
Source: Breast cancer research : BCR   15(6): R106 (Journal)
Registered Authors: He, Shuning, Snaar-Jagalska, Ewa B.
Keywords: none
MeSH Terms:
  • Animals
  • Benzamides/pharmacology
  • Breast Neoplasms/drug therapy
  • Breast Neoplasms/metabolism*
  • Breast Neoplasms/pathology*
  • Chromones/pharmacology
  • Dioxoles/pharmacology
  • Dipeptides/pharmacology
  • Disease Models, Animal
  • Drug Screening Assays, Antitumor/methods
  • Embryo, Nonmammalian
  • Enzyme Inhibitors/pharmacology
  • Female
  • Humans
  • Matrix Metalloproteinase Inhibitors/pharmacology
  • Morpholines/pharmacology
  • Protein-Serine-Threonine Kinases/antagonists & inhibitors
  • RNA, Small Interfering/pharmacology
  • Receptors, Transforming Growth Factor beta/antagonists & inhibitors
  • Signal Transduction/drug effects
  • Smad2 Protein/metabolism
  • Smad4 Protein/genetics
  • Smad4 Protein/metabolism
  • Small Molecule Libraries/pharmacology
  • Transforming Growth Factor beta/antagonists & inhibitors
  • Transforming Growth Factor beta/metabolism*
  • Xenograft Model Antitumor Assays*
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 24196484 Full text @ Breast Cancer Res.
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ABSTRACT

Introduction

The transforming growth factor beta (TGF-β) signalling pathway is known to control human breast cancer invasion and metastasis. We demonstrate that the zebrafish xenograft assay is a robust and dependable animal model for examining the role of pharmacological modulators and genetic perturbation of TGF-β signalling in human breast tumour cells.

Methods

We injected cancer cells into the embryonic circulation (duct of cuvier) and examined their invasion and metastasis into the avascular collagenous tail. Various aspects of the TGF-β signalling pathway were blocked by chemical inhibition, small interfering RNA (siRNA), or small hairpin RNA (shRNA). Analysis was conducted using fluorescent microscopy.

Results

Breast cancer cells with different levels of malignancy, according to in vitro and in vivo mouse studies, demonstrated invasive and metastatic properties within the embryonic zebrafish model that nicely correlated with their differential tumourigenicity in mouse models. Interestingly, MCF10A M2 and M4 cells invaded into the caudal hematopoietic tissue and were visible as a cluster of cells, whereas MDA MB 231 cells invaded into the tail fin and were visible as individual cells. Pharmacological inhibition with TGF-β receptor kinase inhibitors or tumour specific Smad4 knockdown disturbed invasion and metastasis in the zebrafish xenograft model and closely mimicked the results we obtained with these cells in a mouse metastasis model. Inhibition of matrix metallo proteinases, which are induced by TGF-β in breast cancer cells, blocked invasion and metastasis of breast cancer cells.

Conclusions

The zebrafish-embryonic breast cancer xenograft model is applicable for the mechanistic understanding, screening and development of anti-TGF-β drugs for the treatment of metastatic breast cancer in a timely and cost-effective manner.

ADDITIONAL INFORMATION