ZFIN ID: ZDB-PUB-131113-11
Genomic editing opens new avenues for zebrafish as a model for neurodegeneration
Schmid, B., and Haass, C.
Date: 2013
Source: Journal of neurochemistry   127(4): 461-470 (Review)
Registered Authors: Haass, Christian, Schmid, Bettina
Keywords: CRISPR/Cas (clustered regularity interpsaced short palindromic repeats), genomics, neurodegeneration, TALEN, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Deoxyribonucleases/genetics
  • Disease Models, Animal*
  • Gene Knock-In Techniques
  • Gene Knockout Techniques
  • Genetic Engineering/methods*
  • Genome*
  • Humans
  • Neurodegenerative Diseases/genetics*
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed: 24117801 Full text @ J. Neurochem.

Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approaches.