PUBLICATION

The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function

Authors
Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A.
ID
ZDB-PUB-130905-5
Date
2013
Source
Developmental Biology   382(2): 400-412 (Journal)
Registered Authors
Chandrasekhar, Anand, Huang, Peng, Kawakami, Koichi, Pan, Xiufang, Sittaramane, Vinoth
Keywords
hindbrain, facial branchiomotor neuron, floor plate cells, Van gogh-like 2, neuronal migration, cilia
MeSH Terms
  • Gene Expression Regulation, Developmental*
  • Animals
  • Motor Neurons/metabolism*
  • Mice
  • Cell Movement
  • Kruppel-Like Transcription Factors/genetics
  • Kruppel-Like Transcription Factors/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Rhombencephalon/cytology
  • Rhombencephalon/metabolism*
  • Neurogenesis
  • Cilia/physiology*
  • Cell Polarity
  • Membrane Proteins/genetics*
  • Membrane Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism
(all 18)
PubMed
23988578 Full text @ Dev. Biol.
Abstract

Vangl2, a core component of the Planar Cell Polarity pathway, is necessary for the caudal migration of Facial Branchiomotor (FBM) neurons in the vertebrate hindbrain. Studies in zebrafish suggest that vangl2 functions largely non-cell autonomously to regulate FBM neuron migration out of rhombomere 4 (r4), but the cell-type within which it acts is not known. Here, we demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration. Furthermore, FBM neurons fail to migrate caudally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role for this cell type in neuronal migration. Although hindbrain floor plate cilia are disorganized in vangl2 mutant embryos, cilia appear to be dispensable for neuronal migration. Notably, Vangl2 is enriched in the basolateral, but not apical, membranes of floor plate cells. Taken together, our data suggest strongly that Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.

Genes / Markers
Figures
Figure Gallery (17 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ch100TgTransgenic Insertion
    nksagff187aGtTransgenic Insertion
      nkuasgfp1aTgTransgenic Insertion
        rw0TgTransgenic Insertion
          tc240
            Insertion
            te370a
              Point Mutation
              ts294e
                Point Mutation
                tz288
                  Point Mutation
                  zou001TgTransgenic Insertion
                    zou002TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      sox32MO1-sox32MRPHLNO
                      tbxtaMO1-tbxtaMRPHLNO
                      vangl2MO1-vangl2MRPHLNO
                      1 - 3 of 3
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                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GFPEFGGFP
                      mRFPEFGmRFP
                      1 - 3 of 3
                      Show
                      Mapping
                      No data available