PUBLICATION

Zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis

Authors
Prajsnar, T.K., Renshaw, S.A., Ogryzko, N.V., Foster, S.J., Serror, P., and Mesnage, S.
ID
ZDB-PUB-130905-31
Date
2013
Source
Infection and Immunity   81(11): 4271-4279 (Journal)
Registered Authors
Ogryzko, Nikolay, Prajsnar, Thomasz, Renshaw, Steve A.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Disease Models, Animal*
  • Enterococcus faecalis/pathogenicity*
  • Gram-Positive Bacterial Infections/microbiology*
  • Gram-Positive Bacterial Infections/pathology*
  • Immune Evasion
  • Immunocompromised Host
  • Opportunistic Infections/microbiology
  • Opportunistic Infections/pathology
  • Survival Analysis
  • Virulence
  • Whole Body Imaging
  • Zebrafish/microbiology*
PubMed
24002065 Full text @ Infect. Immun.
Abstract

Enterococcus faecalis is an opportunistic pathogen responsible for a wide range of life-threatening nosocomial infections such as septicaemia, peritonitis or endocarditis. E. faecalis infections are associated with a high mortality and substantial healthcare costs and cause therapeutic problems due to the intrinsic resistance of this bacterium to antibiotics. Several factors contributing to E. faecalis virulence have been identified. Due to the variety of infections caused by this organism, numerous animal models have been used to mimic E. faecalis infections, but none of them is considered as ideal to follow the pathogenesis. Here, we studied for the first time E. faecalis pathogenesis in zebrafish larvae. Using model strains, chosen isogenic mutants and fluorescent derivatives expressing GFP, we analyzed both lethality and bacterial dissemination in infected larvae. Genetically engineered immunocompromized zebrafish allowed the identification of two critical steps for successful establishment of disease: i) host phagocytosis evasion mediated by the Epa rhamnopolysaccharide and ii) tissue damage mediated by the quorum sensing Fsr regulon. Our results reveal that zebrafish is a novel, powerful model to study E. faecalis pathogenesis, enabling to dissect the mechanism of enterococcal virulence.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes