PUBLICATION

Small molecule screening in zebrafish: swimming in potential drug therapies

Authors
Tamplin, O.J., White, R.M., Jing, L., Kaufman, C.K., Lacadie, S.A., Li, P., Taylor, A.M., and Zon, L.I.
ID
ZDB-PUB-130710-75
Date
2012
Source
Wiley Interdisciplinary Reviews: Developmental Biology   1(3): 459-468 (Review)
Registered Authors
Jing, Lili, Tamplin, Owen, Taylor, Alison, White, Richard M., Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism*
  • Genetic Testing*
  • Phenotype
  • Small Molecule Libraries/pharmacology*
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
23801494 Full text @ Wiley Interdiscip. Rev. Dev. Biol.
Abstract

Phenotype-driven chemical genetic screens in zebrafish have become a proven approach for both dissection of developmental mechanisms and discovery of potential therapeutics. A library of small molecules can be arrayed into multiwell plates containing zebrafish embryos. The embryo becomes a whole organism in vivo bioassay that can produce a phenotype upon treatment. Screens have been performed that are based simply on the morphology of the embryo. Other screens have scored complex phenotypes using whole mount in situ hybridization, fluorescent transgenic reporters, and even tracking of embryo movement. The availability of many well-characterized zebrafish mutants has also enabled the discovery of chemical suppressors of genetic phenotypes. Importantly, the application of chemical libraries that already contain FDA-approved drugs has allowed the rapid translation of hits from zebrafish chemical screens to clinical trials.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes