PUBLICATION
High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart
- Authors
- Park, J., Cummins, T.M., Harrison, M., Lee, J., Zhou, Q., Lien, C.L., and Shung, K.K.
- ID
- ZDB-PUB-130710-33
- Date
- 2013
- Source
- Optics express 21(12): 14636-14642 (Journal)
- Registered Authors
- Harrison, Michael, Lien, Ching-Ling (Ellen)
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Flow Velocity/physiology
- Coronary Circulation/physiology*
- Echocardiography/instrumentation*
- Elasticity Imaging Techniques/instrumentation*
- Equipment Design
- Equipment Failure Analysis
- Myocardial Perfusion Imaging/instrumentation*
- Photoacoustic Techniques/instrumentation*
- Reproducibility of Results
- Rheology/instrumentation*
- Sensitivity and Specificity
- Zebrafish/anatomy & histology
- Zebrafish/physiology*
- PubMed
- 23787651 Full text @ Opt. Express
Citation
Park, J., Cummins, T.M., Harrison, M., Lee, J., Zhou, Q., Lien, C.L., and Shung, K.K. (2013) High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart. Optics express. 21(12):14636-14642.
Abstract
A technique on high frame rate(28fps), high frequency co-registered ultrasound and photoacoustic imaging for visualizing zebrafish heart blood flow was demonstrated. This approach was achieved with a 40MHz light weight(0.38g) ring-type transducer, serving as the ultrasound transmitter and receiver, to allow an optic fiber, coupled with a 532nm laser, to be inserted into the hole. From the wire target study, axial resolutions of 38µm and 42µm were obtained for ultrasound and photoacoustic imaging, respectively. Carbon nanotubes were utilized as contrast agents to increase the flow signal level by 20dB in phantom studies, and zebrafish heart blood flow was successfully observed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping