ZFIN ID: ZDB-PUB-130610-19
TALE nuclease engineering and targeted genome modification
Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B.
Date: 2013
Source: Yi chuan = Hereditas   35(4): 395-409 (Journal)
Registered Authors: Huang, Peng, Shen, Yan, Xiao, An, Zhang, Bo
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Endonucleases/chemistry
  • Endonucleases/genetics*
  • Endonucleases/metabolism
  • Gene Targeting/methods*
  • Genome/genetics*
  • Humans
  • Molecular Sequence Data
  • Protein Engineering/methods*
  • Recombinant Fusion Proteins/chemistry
  • Recombinant Fusion Proteins/genetics*
  • Recombinant Fusion Proteins/metabolism
PubMed: 23659930 Full text @ Yi Chuan
Artificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.
Article is in Chinese.