PUBLICATION
TALE nuclease engineering and targeted genome modification
- Authors
- Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B.
- ID
- ZDB-PUB-130610-19
- Date
- 2013
- Source
- Yi chuan = Hereditas 35(4): 395-409 (Journal)
- Registered Authors
- Huang, Peng, Shen, Yan, Xiao, An, Zhang, Bo
- Keywords
- none
- MeSH Terms
-
- Protein Engineering/methods*
- Genome/genetics*
- Gene Targeting/methods*
- Animals
- Molecular Sequence Data
- Recombinant Fusion Proteins/chemistry
- Recombinant Fusion Proteins/genetics*
- Recombinant Fusion Proteins/metabolism
- Amino Acid Sequence
- Base Sequence
- Endonucleases/chemistry
- Endonucleases/genetics*
- Endonucleases/metabolism
- Humans
- PubMed
- 23659930 Full text @ Yi Chuan
Citation
Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B. (2013) TALE nuclease engineering and targeted genome modification. Yi chuan = Hereditas. 35(4):395-409.
Abstract
Artificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.
Errata / Notes
Article is in Chinese.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping