PUBLICATION
Assessment of Genotoxicity in Gonads, Liver and Gills of Zebrafish (Danio rerio) by Use of the Comet Assay and Micronucleus Test after In Vivo Exposure to Methyl Methanesulfonate
- Authors
- Faßbender, C., and Braunbeck, T.
- ID
- ZDB-PUB-130507-10
- Date
- 2013
- Source
- Bulletin of environmental contamination and toxicology 91(1): 89-95 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- genotoxicity, zebrafish, comet assay, micronucleus test, gonads, methyl methanesulfonate
- MeSH Terms
-
- Animals
- Comet Assay
- Environmental Monitoring/methods*
- Female
- Gills/drug effects
- Gills/metabolism
- Liver/drug effects
- Liver/metabolism
- Male
- Methyl Methanesulfonate/toxicity*
- Micronucleus Tests
- Ovary/drug effects*
- Ovary/metabolism
- Testis/drug effects*
- Testis/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- PubMed
- 23620131 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Faßbender, C., and Braunbeck, T. (2013) Assessment of Genotoxicity in Gonads, Liver and Gills of Zebrafish (Danio rerio) by Use of the Comet Assay and Micronucleus Test after In Vivo Exposure to Methyl Methanesulfonate. Bulletin of environmental contamination and toxicology. 91(1):89-95.
Abstract
Since generative tissues are a link between the generations, the detection of genetic damage in testis and ovary of fish is conductive to elucidating the relationship between genotoxicity and impairment of reproduction. In the current study, exposure of zebrafish to methyl methanesulfonate over two weeks caused concentration dependent genotoxic effects in gonads, liver and gills using the alkaline comet assay. Likewise, the micronucleus frequency was elevated in all of these organs. Thus, the comet assay and the micronucleus test proved appropriate for the detection of genotoxicity in primary male and female gonad cells and histological sections of the gonads from zebrafish, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping