Scratch2 Prevents Cell Cycle Re-Entry by Repressing miR-25 in Postmitotic Primary Neurons
- Authors
- Rodríguez-Aznar, E., Barrallo-Gimeno, A., and Nieto, M.A.
- ID
- ZDB-PUB-130408-9
- Date
- 2013
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 33(12): 5095-5105 (Journal)
- Registered Authors
- Barrallo Gimeno, Alejandro, Nieto, Angela, Rodríguez-Aznar, Eva
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology
- Cell Survival/physiology
- Cyclin-Dependent Kinase Inhibitor p57/genetics
- Cyclin-Dependent Kinase Inhibitor p57/metabolism
- Gene Expression Regulation, Developmental/physiology
- Gene Knockdown Techniques
- Green Fluorescent Proteins/genetics
- Homeostasis/physiology
- Luminescent Proteins/genetics
- MicroRNAs/genetics
- MicroRNAs/physiology*
- Mitosis/physiology*
- Neurogenesis/physiology
- Neurons/cytology
- Neurons/physiology*
- Spinal Cord/cytology
- Spinal Cord/embryology
- Spinal Cord/physiology
- Transcription Factors/genetics*
- Transcription Factors/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 23516276 Full text @ J. Neurosci.
During the development of the nervous system the regulation of cell cycle, differentiation, and survival is tightly interlinked. Newly generated neurons must keep cell cycle components under strict control, as cell cycle re-entry leads to neuronal degeneration and death. However, despite their relevance, the mechanisms controlling this process remain largely unexplored. Here we show that Scratch2 is involved in the control of the cell cycle in neurons in the developing spinal cord of the zebrafish embryo. scratch2 knockdown induces postmitotic neurons to re-enter mitosis. Scratch2 prevents cell cycle re-entry by maintaining high levels of the cycle inhibitor p57 through the downregulation of miR-25. Thus, Scratch2 appears to safeguard the homeostasis of postmitotic primary neurons by preventing cell cycle re-entry.