PUBLICATION

Whole-brain functional imaging at cellular resolution using light-sheet microscopy

Authors
Ahrens, M.B., Orger, M.B., Robson, D.N., Li, J.M., and Keller, P.J.
ID
ZDB-PUB-130408-26
Date
2013
Source
Nature Methods   10(5): 413-20 (Journal)
Registered Authors
Ahrens, Misha, Keller, Philipp
Keywords
none
MeSH Terms
  • Animals
  • Brain/metabolism
  • Brain/physiology*
  • Microscopy/methods*
  • Zebrafish
PubMed
23524393 Full text @ Nat. Methods
Abstract

Brain function relies on communication between large populations of neurons across multiple brain areas, a full understanding of which would require knowledge of the time-varying activity of all neurons in the central nervous system. Here we use light-sheet microscopy to record activity, reported through the genetically encoded calcium indicator GCaMP5G, from the entire volume of the brain of the larval zebrafish in vivo at 0.8 Hz, capturing more than 80% of all neurons at single-cell resolution. Demonstrating how this technique can be used to reveal functionally defined circuits across the brain, we identify two populations of neurons with correlated activity patterns. One circuit consists of hindbrain neurons functionally coupled to spinal cord neuropil. The other consists of an anatomically symmetric population in the anterior hindbrain, with activity in the left and right halves oscillating in antiphase, on a timescale of 20 s, and coupled to equally slow oscillations in the inferior olive.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping