Henry, K.M., Loynes, C.A., Whyte, M.K., and Renshaw, S.A. (2013) Zebrafish as a model for the study of neutrophil biology. Journal of Leukocyte Biology. 94(4):633-642.
To understand inflammation and immunity, we need to understand the biology of the neutrophil. Whereas these cells can readily
be extracted from peripheral blood, their short lifespan makes genetic manipulations impractical. Murine knockout models have
been highly informative, and new imaging techniques are allowing neutrophils to be seen during inflammation in vivo for the
first time. However, there is a place for a new model of neutrophil biology, which readily permits imaging of individual neutrophils
during inflammation in vivo, combined with the ease of genetic and chemical manipulation. The zebrafish has long been the
model of choice for the developmental biology community, and the availability of genomic resources and tools for gene manipulation
makes this an attractive model. Zebrafish innate immunity shares many features with mammalian systems, including neutrophils
with morphological, biochemical, and functional features, also shared with mammalian neutrophils. Transgenic zebrafish with
neutrophils specifically labeled with fluorescent proteins have been generated, and this advance has led to the adoption of
zebrafish, alongside existing models, by a number of groups around the world. The use of these models has underpinned a number
of key advances in the field, including the identification of a tissue gradient of hydrogen peroxide for neutrophil recruitment
following tissue injury and direct evidence for reverse migration as a regulatable mechanism of inflammation resolution. In
this review, we discuss the importance of zebrafish models in neutrophil biology and describe how the understanding of neutrophil
biology has been advanced by the use of these models.
No data available
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.