PUBLICATION

Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis

Authors
Ellis, K., Bagwell, J., and Bagnat, M.
ID
ZDB-PUB-130322-23
Date
2013
Source
The Journal of cell biology   200(5): 667-679 (Journal)
Registered Authors
Bagnat, Michel
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axis, Cervical Vertebra/embryology
  • Axis, Cervical Vertebra/metabolism
  • Axis, Cervical Vertebra/physiology*
  • Cell Movement
  • Endocytosis
  • Endosomes/metabolism
  • Gene Expression Regulation, Developmental
  • HEK293 Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Lysosomes/metabolism
  • Lysosomes/physiology*
  • Microscopy, Confocal
  • Morphogenesis
  • Notochord/metabolism
  • Notochord/physiology*
  • Protein Transport
  • Proton-Translocating ATPases
  • Recombinant Fusion Proteins/metabolism
  • Spine/embryology
  • Spine/metabolism
  • Spine/physiology*
  • Time Factors
  • Time-Lapse Imaging
  • Transfection
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
  • rab GTP-Binding Proteins/metabolism
PubMed
23460678 Full text @ J. Cell Biol.
Abstract

The notochord plays critical structural and signaling roles during vertebrate development. At the center of the vertebrate notochord is a large fluid-filled organelle, the notochord vacuole. Although these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenance. Here we show that zebrafish notochord vacuoles are specialized lysosome-related organelles whose formation and maintenance requires late endosomal trafficking regulated by the vacuole-specific Rab32a and H-ATPase-dependent acidification. We establish that notochord vacuoles are required for body axis elongation during embryonic development and identify a novel role in spine morphogenesis. Thus, the vertebrate notochord plays important structural roles beyond early development.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes