ZFIN ID: ZDB-PUB-130309-22
TALEN-mediated precise genome modification by homologous recombination in zebrafish
Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., and Lin, S.
Date: 2013
Source: Nature Methods   10(4): 329-31 (Journal)
Registered Authors: Huang, Peng, Hu, Yingying, Lin, Shuo, Luo, Zhou, Tong, Xiangjun, Wang, Zhanxiang, Wu, Qian, Zhang, Bo, Zhu, Zuoyan, Zu, Yao
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Deoxyribonucleases/genetics
  • Deoxyribonucleases/metabolism*
  • Exons
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Targeting/methods
  • Genetic Engineering/methods
  • Genomics
  • RNA
  • Recombination, Genetic/physiology*
  • Tyrosine 3-Monooxygenase/genetics
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 23435258 Full text @ Nat. Methods

We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator–like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.