PUBLICATION

In vivo imaging of disease-related mitochondrial dynamics in a vertebrate model system

Authors
Plucinska, G., Paquet, D., Hruscha, A., Godinho, L., Haass, C., Schmid, B., and Misgeld, T.
ID
ZDB-PUB-121205-1
Date
2012
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   32(46): 16203-16212 (Journal)
Registered Authors
Godinho, Leanne, Haass, Christian, Hruscha, Alexander, Paquet, Dominik, Schmid, Bettina
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Antineoplastic Agents/pharmacology
  • Biological Transport/physiology
  • Blotting, Western
  • Image Processing, Computer-Assisted
  • Mitochondria/pathology*
  • Mitochondria/ultrastructure
  • Nervous System Diseases/pathology*
  • Nocodazole/pharmacology
  • Phenotype
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/genetics
  • Sensory Receptor Cells/physiology
  • Sensory Receptor Cells/ultrastructure
  • Zebrafish/physiology*
  • tau Proteins/genetics
PubMed
23152604 Full text @ J. Neurosci.
Abstract

Mitochondria provide ATP, maintain calcium homeostasis, and regulate apoptosis. Neurons, due to their size and complex geometry, are particularly dependent on the proper functioning and distribution of mitochondria. Thus disruptions of these organelles and their transport play a central role in a broad range of neurodegenerative diseases. While in vitro studies have greatly expanded our knowledge of mitochondrial dynamics, our understanding in vivo remains limited. To address this shortcoming, we developed tools to study mitochondrial dynamics in vivo in optically accessible zebrafish. We demonstrate here that our newly generated tools, including transgenic “MitoFish,” can be used to study the in vivo “life cycle” of mitochondria and allows identifying pharmacological and genetic modulators of mitochondrial dynamics. Furthermore we observed profound mitochondrial transport deficits in real time in a zebrafish tauopathy model. By rescuing this phenotype using MARK2 (microtubule-affinity regulating kinase 2), we provide direct in vivo evidence that this kinase regulates axonal transport in a Tau-dependent manner. Thus, our approach allows detailed studies of the dynamics of mitochondria in their natural environment under normal and disease conditions.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes