Abdallah, S.J., Perry, S.F., and Jonz, M.G. (2012) CO(2) Signaling in Chemosensory Neuroepithelial Cells of the Zebrafish Gill Filaments: Role of Intracellular Ca(2+) and pH. Advances in experimental medicine and biology. 758:143-148.
Adult zebrafish, Danio rerio, exhibit hyperventilatory responses to absolute environmental CO2 levels as low as 1.0 mmHg. The ability of zebrafish to
detect and respond to low ambient CO2 appears to be mediated by chemosensory neuroepithelial cells (NECs) of the gill filaments.
Recent electrophysiological characterization of this response revealed that the partial pressure-dependent depolarization
of NECs in response to a hypercapnic stimulus is dependent on the rate of acidification associated with the hydration of CO2
and the inhibition of background K+ channels. In order to further elucidate the signaling pathway underlying CO2 chemotransduction in NECs, we used microfluorimetric
techniques to study intracellular changes in pH (pHi) and calcium ([Ca2+]i). Using the ratiometric indicators BCECF-AM and fura-2-AM, we found that a hypercapnic stimulus evoked a decrease in pHi
and an increase in [Ca2+]i.
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