PUBLICATION

Biasing Amacrine Subtypes in the Atoh7 Lineage through Expression of Barhl2

Authors
Jusuf, P.R., Albadri, S., Paolini, A., Currie, P.D., Argenton, F., Higashijima, S., Harris, W.A., and Poggi, L.
ID
ZDB-PUB-121016-1
Date
2012
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   32(40): 13929-13944 (Journal)
Registered Authors
Albadri, Shahad, Argenton, Francesco, Currie, Peter D., Harris, William A., Higashijima, Shin-ichi, Jusuf, Patricia, Paolini, Alessio, Poggi, Lucia
Keywords
none
MeSH Terms
  • Amacrine Cells/classification
  • Amacrine Cells/cytology*
  • Amacrine Cells/metabolism
  • Animals
  • Animals, Genetically Modified
  • Cell Division
  • Cell Lineage
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology*
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Helix-Loop-Helix Motifs/physiology
  • Male
  • Morpholinos/pharmacology
  • Retina/embryology
  • Time-Lapse Imaging
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Transcription, Genetic/drug effects
  • Zebrafish
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
23035102 Full text @ J. Neurosci.
Abstract

Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes