Embryonic Fate Map of First Pharyngeal Arch Structures in the sox10: kaede Zebrafish Transgenic Model
- Authors
- Dougherty, M., Kamel, G., Shubinets, V., Hickey, G., Grimaldi, M., and Liao, E.C.
- ID
- ZDB-PUB-120907-5
- Date
- 2012
- Source
- The Journal of craniofacial surgery 23(5): 1333-1337 (Journal)
- Registered Authors
- Liao, Eric
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Branchial Region/embryology*
- Embryo, Nonmammalian/metabolism
- Estradiol/pharmacology
- Gene Expression Regulation, Developmental
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism
- In Situ Hybridization
- Luminescent Proteins/genetics*
- Luminescent Proteins/metabolism
- MicroRNAs/genetics
- MicroRNAs/metabolism
- Morphogenesis/genetics
- Neural Crest/embryology
- SOXE Transcription Factors/genetics
- SOXE Transcription Factors/metabolism
- Signal Transduction
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 22948622 Full text @ J. Craniofac. Surg.
Cranial neural crest cells follow stereotypic patterns of migration to form craniofacial structures. The zebrafish is a powerful vertebrate genetic model where transgenics with reporter proteins under the transcriptional regulation of lineage-specific promoters can be generated. Numerous studies demonstrate that the zebrafish ethmoid plate is embryologically analogous to the mammalian palate. A fate map correlating embryonic cranial neural crest to defined jaw structures would provide a useful context for the morphogenetic analysis of craniofacial development. To that end, the sox10:kaede transgenic was generated, where sox10 provides lineage restriction to the neural crest. Specific regions of neural crest were labeled at the 10-somite stage by photoconversion of the kaede reporter protein. Lineage analysis was carried out during pharyngeal development in wild-type animals, after miR140 injection, and after estradiol treatment. At the 10-somite stage, cranial neural crest cells anterior of the eye contributed to the median ethmoid plate, whereas cells medial to the eye formed the lateral ethmoid plate and trabeculae and a posterior population formed the mandible. miR-140 overexpression and estradiol inhibition of Hedgehog signaling resulted in cleft development, with failed migration of the anterior cell population to form the median ethmoid plate. The sox10:kaede transgenic line provides a useful tool for neural crest lineage analysis. These studies illustrate the advantages of the zebrafish model for application in morphogenetic studies of vertebrate craniofacial development.