Mechanism of cadmium-induced cytotoxicity on the ZFL zebrafish liver cell line
- Authors
- Zhu, J.Y., and Chan, K.M.
- ID
- ZDB-PUB-120905-14
- Date
- 2012
- Source
- Metallomics : integrated biometal science 4(10): 1064-1076 (Journal)
- Registered Authors
- Chan, King-Ming
- Keywords
- none
- MeSH Terms
-
- Analysis of Variance
- Animals
- Antioxidants/metabolism
- Cadmium/metabolism
- Cadmium/toxicity*
- Cell Line
- Electrophoresis, Gel, Two-Dimensional
- Flow Cytometry
- Hepatocytes/chemistry
- Hepatocytes/drug effects*
- Hepatocytes/enzymology
- Hepatocytes/metabolism*
- Intracellular Space/chemistry
- Intracellular Space/drug effects
- Oxidoreductases/genetics
- Oxidoreductases/metabolism
- Proteome/analysis
- Proteome/drug effects
- Proteomics
- Reactive Oxygen Species/analysis
- Reactive Oxygen Species/metabolism
- Zebrafish
- PubMed
- 22941245 Full text @ Metallomics
- CTD
- 22941245
The cadmium ion (Cd2+) is a highly toxic metal ion; however, its hepatic toxic effects are not very well characterized in a systematic manner. In this study, a zebrafish liver cell line, ZFL was used as a model to investigate the mechanism of Cd2+-induced cytotoxicity on hepatocytes. The intracellular level of reactive oxygen species decreased following the administration of Cd2+; antioxidant levels and related enzyme activities and gene expression were detected, showing that the toxic effects of Cd2+ might not be coupled to oxidative stress. To understand the cytotoxic effects of Cd2+ on ZFL cells after Cd2+ exposure, a total of 77 differentially expressed proteins were detected by two-dimensional gel electrophoresis; 43 of them were further identified by MALDI-TOF-MS. The proteins that responded to Cd2+ in ZFL cells were related to stress response, transporters, regulation of transcription, redox homeostasis, or different signaling pathways, with half of these proteins having metal ion binding capabilities.