Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues
- Authors
- Verstraeten, B., Sanders, E., and Huysseune, A.
- ID
- ZDB-PUB-120510-19
- Date
- 2012
- Source
- Methods in molecular biology (Clifton, N.J.) 887: 179-191 (Chapter)
- Registered Authors
- Huysseune, Ann
- Keywords
- tooth development, tooth replacement, zebrafish, whole mount immunohistochemistry, whole mount, in situ hybridization, epon embedding, decalcification
- MeSH Terms
-
- Animals
- Immunohistochemistry/methods*
- In Situ Hybridization/methods*
- Larva/metabolism*
- Tooth/metabolism
- Zebrafish/metabolism*
- PubMed
- 22566056 Full text @ Meth. Mol. Biol.
Tooth development is increasingly being studied in a variety of vertebrate model organisms, each contributing its own perspective to our understanding of dental diversity. In situ hybridization and immunohistochemistry are well-established and frequently used techniques to study the presence of mRNA and protein. Here, we describe a protocol for whole mount immunohistochemistry and in situ hybridization that can be applied to all stages of zebrafish development and dissected bony parts. The description of these protocols is followed by the outline of a quick decalcification method and the procedure for embedding in epoxy resin to obtain serial sections with high histological quality.