ZFIN ID: ZDB-PUB-120417-5
Epidermal growth factor differentially regulates activin subunits in the zebrafish ovarian follicle cells via diverse signaling pathways
Chung, C.K., and Ge, W.
Date: 2012
Source: Molecular and Cellular Endocrinology   361(1-2): 133-142 (Journal)
Registered Authors: Ge, Wei
Keywords: EGF, activin, ovary, zebrafish
MeSH Terms:
  • Animals
  • Cells, Cultured
  • Epidermal Growth Factor/pharmacology*
  • Female
  • Humans
  • Inhibin-beta Subunits/genetics
  • Inhibin-beta Subunits/metabolism*
  • MAP Kinase Signaling System/drug effects
  • Models, Biological
  • Ovarian Follicle/cytology*
  • Ovarian Follicle/drug effects
  • Ovarian Follicle/enzymology
  • Ovarian Follicle/metabolism*
  • Phosphatidylinositol 3-Kinases/metabolism
  • Protein Kinases/metabolism
  • Recombinant Proteins/pharmacology
  • Signal Transduction/drug effects*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • p38 Mitogen-Activated Protein Kinases/metabolism
PubMed: 22503865 Full text @ Mol. Cell. Endocrinol.

Epidermal growth factor (EGF) promotes oocyte maturation in the zebrafish and its effect is mediated via the activin system. However, the mechanisms by which EGF regulates activin subunits in the follicle cells remain unknown. The present study demonstrated that EGF controlled expression of three activin subunits (inhbaa, inhbab and inhbb) in the follicle cells via diverse signaling pathways. The expression of inhbaa and inhbb was often co-regulated via similar pathways. Suppression of MAPK3/1, p38 MAPK, PKC and PKA each blocked or partially reduced the stimulatory effects of EGF on the expression of inhbaa and inhbb while up-regulated that of inhbab. Conversely, inhibition of PI3K did not have any effect on the expression of inhbaa and inhbb but significantly suppressed the stimulatory effect of EGF on inhbab. In summary, EGF action in the zebrafish ovary involves activin system and its regulation of activin subunits is mediated by diverse signaling pathways downstream of EGFR.