PUBLICATION

PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration

Authors
Dempsey, W.P., Fraser, S.E., and Pantazis, P.
ID
ZDB-PUB-120326-22
Date
2012
Source
PLoS One   7(3): e32888 (Journal)
Registered Authors
Dempsey, William, Fraser, Scott E., Pantazis, Periklis (Laki)
Keywords
none
MeSH Terms
  • Animal Fins/physiology
  • Animals
  • Animals, Genetically Modified/embryology*
  • Animals, Genetically Modified/physiology*
  • Cell Division
  • Cell Lineage*
  • Cell Membrane/metabolism
  • Cell Nucleus/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Gastrulation/physiology
  • Genetic Vectors/genetics
  • Regeneration/physiology*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/physiology*
PubMed
22431986 Full text @ PLoS One
Abstract

Background

Elucidating the complex cell dynamics (divisions, movement, morphological changes, etc.) underlying embryonic development and adult tissue regeneration requires an efficient means to track cells with high fidelity in space and time. To satisfy this criterion, we developed a transgenic zebrafish line, called PhOTO, that allows photoconvertible optical tracking of nuclear and membrane dynamics in vivo.

Methodology

PhOTO zebrafish ubiquitously express targeted blue fluorescent protein (FP) Cerulean and photoconvertible FP Dendra2 fusions, allowing for instantaneous, precise targeting and tracking of any number of cells using Dendra2 photoconversion while simultaneously monitoring global cell behavior and morphology. Expression persists through adulthood, making the PhOTO zebrafish an excellent tool for studying tissue regeneration: after tail fin amputation and photoconversion of a ~100μm stripe along the cut area, marked differences seen in how cells contribute to the new tissue give detailed insight into the dynamic process of regeneration. Photoconverted cells that contributed to the regenerate were separated into three distinct populations corresponding to the extent of cell division 7 days after amputation, and a subset of cells that divided the least were organized into an evenly spaced, linear orientation along the length of the newly regenerating fin.

Conclusions/Significance

PhOTO zebrafish have wide applicability for lineage tracing at the systems-level in the early embryo as well as in the adult, making them ideal candidate tools for future research in development, traumatic injury and regeneration, cancer progression, and stem cell behavior.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes