PUBLICATION
Quantitative assessment of the embryotoxic potential of NSO-heterocyclic compounds using zebrafish (Danio rerio)
- Authors
- Peddinghaus, S., Brinkmann, M., Bluhm, K., Sagner, A., Hinger, G., Braunbeck, T., Eisenträger, A., Tiehm, A., Hollertm, H., and Keiter, S.H.
- ID
- ZDB-PUB-120117-19
- Date
- 2012
- Source
- Reproductive toxicology (Elmsford, N.Y.) 33(2): 224-232 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- heterocyclic aromatic compounds, NSO-HET, zebrafish, embryo toxicity, fish embryo toxicity test
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects
- Environmental Pollutants/toxicity*
- Heterocyclic Compounds/toxicity*
- Lethal Dose 50
- Models, Biological
- Quantitative Structure-Activity Relationship
- Teratogens/toxicity*
- Zebrafish*
- PubMed
- 22244949 Full text @ Reprod. Toxicol.
Citation
Peddinghaus, S., Brinkmann, M., Bluhm, K., Sagner, A., Hinger, G., Braunbeck, T., Eisenträger, A., Tiehm, A., Hollertm, H., and Keiter, S.H. (2012) Quantitative assessment of the embryotoxic potential of NSO-heterocyclic compounds using zebrafish (Danio rerio). Reproductive toxicology (Elmsford, N.Y.). 33(2):224-232.
Abstract
Heterocyclic aromatic compounds (NSO-HET) have frequently been detected in the environment. Several studies have concluded that NSO-HET pose a threat to organisms in waters, sediments and soils. Consequently, several heterocyclic compounds are under discussion to be included in the priority list of the European Water Framework Directive. However, few publications are available assessing the ecotoxicology of NSO-HET. The present study aims to assess the embryo toxicity of heterocycles using Danio rerio. A combination of the Fish Embryo Toxicity Test and analytical quantification should aid to determine the hazard potential. Changes of the total concentrations due to sorption or volatility were quantified by GC/MS. Loss of compounds during the test was observed primarily for volatile or hydrophobic NSO-HET. The LC50 calculated with nominal concentrations underestimates the toxicity by a factor up to 16 (2 h), demonstrating that a chemical analysis for comparing nominal and measured concentrations is essential for such investigations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping