PUBLICATION
Effects of the anti-thyroidal compound potassium-perchlorate on the thyroid system of the zebrafish
- Authors
- Schmidt, F., Schnurr, S., Wolf, R., and Braunbeck, T.
- ID
- ZDB-PUB-120110-3
- Date
- 2012
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 109C: 47-58 (Journal)
- Registered Authors
- Braunbeck, Thomas
- Keywords
- none
- MeSH Terms
-
- Animals
- Antithyroid Agents/toxicity*
- Body Constitution/drug effects
- Body Size/drug effects
- Body Weight/drug effects
- Cell Proliferation/drug effects
- Gonads/drug effects
- Liver/drug effects
- Perchlorates/toxicity*
- Pituitary Gland/drug effects
- Potassium Compounds/toxicity*
- Thyroid Gland/drug effects*
- Thyroxine/analysis
- Water Pollutants, Chemical/toxicity*
- Zebrafish/physiology*
- PubMed
- 22204988 Full text @ Aquat. Toxicol.
Citation
Schmidt, F., Schnurr, S., Wolf, R., and Braunbeck, T. (2012) Effects of the anti-thyroidal compound potassium-perchlorate on the thyroid system of the zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 109C:47-58.
Abstract
The increasing pollution of aquatic habitats with anthropogenic compounds has led to various test strategies to detect hazardous chemicals. However, information on effects of pollutants in the thyroid system in fish, which is essential for growth, development and parts of reproduction, is still scarce. Other vertebrate groups such as amphibians or mammals are well-studied; so the need for further knowledge especially in fish as a favored vertebrate model test organism is evident. Modified early life-stage tests were carried out with zebrafish exposed to the known thyroid inhibitor potassium perchlorate (0, 62.5, 125, 250, 500 and 5000 μg/L) to identify adverse effects on the hypothalamic-pituitary–thyroid axis. Especially higher perchlorate concentrations led to conspicuous alterations in thyroidal tissue architecture and to effects in the pituitary. In the thyroid, severe hyperplasia at concentrations e500 μg/L together with an increase in follicle number could be detected. The most sensitive endpoint was the colloid, which showed alterations at e250 μg/L. The tinctorial properties and the texture of the colloid changed dramatically. Interestingly, effects on epithelial cell height were minor. The pituitary revealed significant proliferations of TSH-producing cells resulting in alterations in the ratio of adeno- to neurohypophysis. The liver as the main site of T4 deiodination showed severe glycogen depletion at concentrations e250 μg/L. In summary, the thyroid system in zebrafish showed effects by perchlorate from concentrations e250 μg/L, thus documenting a high sensitivity of the zebrafish thyroid gland for goitrogens. In the future, such distinct alterations could lead to a better understanding and identification of potential thyroid-disrupting chemicals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping