ZFIN ID: ZDB-PUB-111117-17
Sox9 Is Upstream of MicroRNA-140 in Cartilage
Nakamura, Y., He, X., Kato, H., Wakitani, S., Kobayashi, T., Watanabe, S., Iida, A., Tahara, H., Warman, M.L., Watanapokasin, R., and Postlethwait, J.H.
Date: 2012
Source: Applied Biochemistry and Biotechnology   166(1): 64-71 (Journal)
Registered Authors: He, Xinjun, Postlethwait, John H., Watanabe, Sumiko
Keywords: microRNA-140 (miR-140), Sox9, cartilage, zebrafish, T/C-28, siRNA, in situ hybridization, RT-PCR
MeSH Terms:
  • Animals
  • Binding Sites
  • Cartilage/growth & development*
  • Cartilage/metabolism
  • Chondrocytes/metabolism
  • Chondrogenesis/genetics
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • MicroRNAs/genetics*
  • MicroRNAs/metabolism*
  • Phylogeny
  • Protein Binding
  • RNA, Small Interfering/genetics
  • SOX9 Transcription Factor/genetics*
  • SOX9 Transcription Factor/metabolism*
  • Ubiquitin-Protein Ligases/genetics
  • Zebrafish/genetics
  • Zebrafish/growth & development*
PubMed: 22052544 Full text @ Appl. Biochem. Biotechnol.

MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.