PCR-cloning of tilapia ATP7A cDNA and its mRNA levels in tissues of tilapia following copper administrations
- Authors
- Chen, D.S., and Chan, K.M.
- ID
- ZDB-PUB-111027-5
- Date
- 2011
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 105(3-4): 717-727 (Journal)
- Registered Authors
- Chan, King-Ming
- Keywords
- copper transporters, copper tolerance, copper uptake
- MeSH Terms
-
- Adenosine Triphosphatases/genetics
- Adenosine Triphosphatases/metabolism*
- Animals
- Base Sequence
- Cloning, Molecular
- Copper/toxicity*
- DNA, Complementary/chemistry
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Regulation/drug effects
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, DNA
- Tilapia/genetics
- Tilapia/metabolism*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21996258 Full text @ Aquat. Toxicol.
- CTD
- 21996258
We are studying the toxicity of copper to tilapia and zebrafish and have found that the copper tolerance of tilapia and the sensitivity of zebrafish were due to several proteins’ regulation mechanisms that were related to the effects of reactive oxygen species, mitochondrion copper transport, and stress response. To further reveal the mechanism of copper tolerance and sensitivity in tilapia and zebrafish, a full length cDNA of ATP7A was obtained in tilapia. Using real time quantitative PCR, the differential regulations of ATP7A in tilapia and zebrafish were studied. It was found that Cu2+ gave a higher induction of ATP7A in tilapia than zebrafish, both in vivo and in vitro. These results suggest that the copper tolerance of tilapia may be due to higher expression level of ATP7A.