ZFIN ID: ZDB-PUB-110921-29
Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish
Pan, C.Y., Wu, J.L., Hui, C.F., Lin, C.H., and Chen, J.Y.
Date: 2011
Source: Fish & shellfish immunology   31(6): 1019-25 (Journal)
Registered Authors: Chen, Jyh-Yih, Wu, Jen-Leih
Keywords: epinecidin-1, vibrio vulnificus
MeSH Terms:
  • Animals
  • Antimicrobial Cationic Peptides/immunology
  • Antimicrobial Cationic Peptides/pharmacology*
  • Antimicrobial Cationic Peptides/therapeutic use
  • DNA Primers/genetics
  • Fish Proteins/immunology
  • Fish Proteins/pharmacology*
  • Fish Proteins/therapeutic use
  • Gene Expression Regulation/drug effects
  • Gene Expression Regulation/immunology*
  • Immunomodulation/immunology*
  • Interferon-gamma/metabolism
  • Interleukin-10/metabolism
  • Interleukin-1beta/metabolism
  • Microarray Analysis
  • Real-Time Polymerase Chain Reaction
  • Survival Analysis
  • Tumor Necrosis Factor-alpha/metabolism
  • Vibrio Infections/immunology*
  • Vibrio Infections/prevention & control*
  • Zebrafish
PubMed: 21925271 Full text @ Fish Shellfish Immunol.
ABSTRACT
In the present study, we used Vibrio vulnificus and a zebrafish model system to investigate the inhibitory effect of epinecidin-1 on acute bacterial infection and studied the impacts of pretreatment, co-treatment, and post-treatment with epinecidin-1 on its protective efficacy. In vivo experiments showed that co-treatment with epinecidin-1 and V. vulnificus achieved 78%–97% survival rates after 30 days. When epinecidin-1 and V. vulnificus were co-injected into zebrafish and zebrafish were re-challenged with V. vulnificus after 30 days, zebrafish had survival rates of 22%–47%. Pretreatment and post-treatment with epinecidin-1 obtained respective survival rates of 57% and 60%. In addition, epinecidin-1 modulated the expressions of immune-responsive genes like interleukin (IL)-10, IL-1b, tumor necrosis factor-α, and interferon-γ as analyzed by a microarray and qPCR approach. This study demonstrates the use of epinecidin-1 to develop inactivated material for fish bacterial infections which can provide guidelines for the future design of epinecidin-1-bacterial formulations for various in vivo applications.
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