PUBLICATION

The role of stat1b in zebrafish hematopoiesis

Authors
Song, H., Yan, Y.L., Titus, T., He, X., and Postlethwait, J.H.
ID
ZDB-PUB-110920-26
Date
2011
Source
Mechanisms of Development   128(7-10): 442-56 (Journal)
Registered Authors
He, Xinjun, Postlethwait, John H., Titus, Tom A., Yan, Yi-Lin
Keywords
Stat1α, Stat1β, hematopoiesis, myeloid, granulocyte, zebrafish
MeSH Terms
  • Synteny/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Hematopoiesis/physiology*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism*
  • Myeloid Cells/metabolism
  • STAT1 Transcription Factor/genetics
  • STAT1 Transcription Factor/metabolism*
  • Animals
  • Phylogeny
  • Cell Lineage
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Gene Duplication
  • Humans
  • Hematopoietic System/embryology
  • Hematopoietic System/metabolism*
  • Biomarkers/metabolism
(all 19)
PubMed
21914475 Full text @ Mech. Dev.
Abstract

STAT1 mediates response to interferons and regulates immunity, cell proliferation, apoptosis, and sensitivity of Fanconi Anemia cells to apoptosis after interferon signaling; the roles of STAT1 in embryos, however, are not understood. To explore embryonic functions of STAT1, we investigated stat1b, an unstudied zebrafish co-ortholog of human STAT1. Zebrafish stat1a encodes all five domains of the human STAT1-alpha splice form but, like the human STAT1-beta splice variant, stat1b lacks a complete transactivation domain; thus, two unlinked zebrafish paralogs encode protein forms translated from two splice variants of a single human gene, as expected by sub functionalization after genome duplication. Phylogenetic and conserved synteny studies showed that stat1b and stat1a arose as duplicates in the teleost genome duplication (TGD) and clarified the evolutionary origin of STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B and STAT6 by tandem and genome duplication. RT-PCR revealed maternal expression of stat1a and stat1b. In situ hybridization detected stat1b but not stat1a expression in embryonic hematopoietic tissues. Morpholino knockdown of stat1b, but not stat1a, decreased expression of the myeloid and granulocyte markers spi and mpo and increased expression of the hematopoietic progenitor marker scl, the erythrocyte marker gata1, and hemoglobin. These results suggest that zebrafish Stat1b promotes myeloid development at the expense of erythroid development.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
stat1aMO1-stat1aMRPHLNO
stat1bMO1-stat1bMRPHLNO
1 - 2 of 2
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Fish
Antibodies
No data available
Orthology
Gene Orthology
stat1a
stat1b
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Engineered Foreign Genes
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Mapping
No data available