The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish
- Authors
- Oehlers, S.H., Flores, M.V., Hall, C.J., Swift, S., Crosier, K.E., and Crosier, P.S.
- ID
- ZDB-PUB-110713-24
- Date
- 2011
- Source
- Disease models & mechanisms 4(6): 832-41 (Journal)
- Registered Authors
- Crosier, Kathy, Flores, Maria, Hall, Chris, Oehlers, Stefan
- Keywords
- none
- MeSH Terms
-
- Neutrophils/metabolism
- Fish Diseases/embryology
- Fish Diseases/microbiology
- Larva/enzymology
- Larva/genetics
- Nod1 Signaling Adaptor Protein/genetics*
- Nod1 Signaling Adaptor Protein/metabolism
- Cloning, Molecular
- Genetic Predisposition to Disease*
- Humans
- Salmonella enterica/physiology
- Nod2 Signaling Adaptor Protein/genetics*
- Nod2 Signaling Adaptor Protein/metabolism
- Embryonic Development
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/microbiology*
- Disease Resistance/genetics
- Inflammatory Bowel Diseases/embryology
- Inflammatory Bowel Diseases/genetics*
- Animals
- RNA Splicing/genetics
- Gene Knockdown Techniques
- Anti-Bacterial Agents/metabolism*
- Sequence Homology, Nucleic Acid
- Signal Transduction/genetics
- NADPH Oxidases
- Gene Expression Regulation, Developmental
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Gastrointestinal Tract/metabolism
- Gastrointestinal Tract/pathology
- PubMed
- 21729873 Full text @ Dis. Model. Mech.
Inflammatory bowel disease (IBD), in the form of Crohn’s disease (CD) or ulcerative colitis (UC), is a debilitating chronic immune disorder of the intestine. A complex etiology resulting from dysfunctional interactions between the intestinal immune system and its microflora, influenced by host genetic susceptibility, makes disease modeling challenging. Mutations in NOD2 have the highest disease-specific risk association for CD, and a related gene, NOD1, is associated with UC. NOD1 and NOD2 encode intracellular bacterial sensor proteins acting as innate immune triggers, and represent promising therapeutic targets. The zebrafish has the potential to aid in modeling genetic and environmental aspects of IBD pathogenesis. Here, we report the characterization of the Nod signaling components in the zebrafish larval intestine. The nod1 and nod2 genes are expressed in intestinal epithelial cells and neutrophils together with the Nod signaling pathway genes ripk2, a20, aamp, cd147, centaurin b1, erbin and grim-19. Using a zebrafish embryo Salmonella infection model, morpholino-mediated depletion of Nod1 or Nod2 reduced the ability of embryos to control systemic infection. Depletion of Nod1 or Nod2 decreased expression of dual oxidase in the intestinal epithelium and impaired the ability of larvae to reduce intracellular bacterial burden. This work highlights the potential use of zebrafish larvae in the study of components of IBD pathogenesis.