PUBLICATION
Alkali-like myosin light chain-1 (myl1) is an early marker for differentiating fast muscle cells in zebrafish
- Authors
- Burguière, A.C., Nord, H., and von Hofsten, J.
- ID
- ZDB-PUB-110628-32
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(7): 1856-1863 (Journal)
- Registered Authors
- Burguière, Anne-Cécile, von Hofsten, Jonas
- Keywords
- alkali-like myosin light chain-1, zebrafish, fast muscle cells
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Immunohistochemistry
- In Situ Hybridization
- Muscle Fibers, Fast-Twitch/metabolism*
- Myosin Light Chains/genetics
- Myosin Light Chains/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21674687 Full text @ Dev. Dyn.
Citation
Burguière, A.C., Nord, H., and von Hofsten, J. (2011) Alkali-like myosin light chain-1 (myl1) is an early marker for differentiating fast muscle cells in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(7):1856-1863.
Abstract
During myogenesis, muscle precursors become divided into either fast- or slow-twitch fibres, which in the zebrafish occupy distinct domains in the embryo. Genes encoding sarcomeric proteins specific for fast or slow fibres are frequently used as lineage markers. In an attempt to identify and evaluate early definitive markers for cells in the fast-twitch pathway, we analysed genes encoding proteins contributing to the fast sarcomeric structures. The previously uncharacterized zebrafish alkali-like myosin light chain gene (myl1) was found to be expressed exclusively in cells in the fast-twitch pathway initiated at an early stage of fast fibre differentiation. Myl1 was expressed earlier, and in a more fibre type restricted manner, than any of the previously described and frequently used fast myosin light and heavy chain and troponin muscle markers mylz2, mylz3, tnni2, tnnt3a, fMyHC1.3. In summary, this study introduces a novel marker for early differentiating fast muscle cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping