PUBLICATION
Laser-induced thrombosis in zebrafish
- Authors
- Jagadeeswaran, P., Carrillo, M., Radhakrishnan, U.P., Rajpurohit, S.K., and Kim, S.
- ID
- ZDB-PUB-110523-5
- Date
- 2011
- Source
- Methods in cell biology 101: 197-203 (Chapter)
- Registered Authors
- Carrillo, Mairo, Jagadeeswaran, Pudur, Kim, Seongcheol, Radhakrishnan, Uvaraj P., Rajpurohit, Surendra
- Keywords
- hemostasis, laser ablation, thrombin, thrombosis, vascular occlusion, vasculature
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Hemostasis
- Humans
- Lasers
- Thrombosis/blood
- Thrombosis/etiology*
- Zebrafish/blood*
- PubMed
- 21550445 Full text @ Meth. Cell. Biol.
Citation
Jagadeeswaran, P., Carrillo, M., Radhakrishnan, U.P., Rajpurohit, S.K., and Kim, S. (2011) Laser-induced thrombosis in zebrafish. Methods in cell biology. 101:197-203.
Abstract
In the event of injury to the vasculature in vertebrate organisms bleeding is stopped by a defense mechanism called hemostasis. Even though biochemical studies characterized a number of factors, classical genetic methods have not been applied to study hemostasis. We introduced zebrafish as an animal model to study genetics of hemostasis. To conduct genetic studies of hemostasis, we required a global screening method to address all the factors of hemostasis such as those present in plasma, in platelets or those present in the endothelium. Therefore, we developed a global laser induced thrombosis method which can assay all these components. In this paper, we describe the principle of this method as well as provide the detailed protocol so this could be used as a screening tool to measure hemostasis in any laboratory.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping