ZFIN ID: ZDB-PUB-110519-15
The transcriptional mediator component med12 is required for hindbrain boundary formation
Hong, S.K., and Dawid, I.B.
Date: 2011
Source: PLoS One   6(4): e19076 (Journal)
Registered Authors: Dawid, Igor B.
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning
  • Cell Proliferation
  • Mediator Complex/genetics
  • Mediator Complex/physiology*
  • Mutation
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 21533047 Full text @ PLoS One


Rhombomere boundaries form during hindbrain segmentation and are critical for maintaining segmental integrity and regulating migration in the hindbrain. Some genetic models affecting hindbrain boundary formation have been described, but involvement of components of the transcriptional mediator complex in boundary formation has not reported so far.

Principal Findings

The kto/med12 mutant zebrafish, which affects the Mediator component Med12, causes specific loss of rhombomere boundary cells even though segmentation of the hindbrain takes place at least in part. In kto mutant embryos, cells forming rhombomere boundaries were largely absent as indicated by the use of several marker genes. While no obvious increase in cell death was observed, we found a notable reduction of cell proliferation in the hindbrain of kto mutant zebrafish.


The kto/med12 mutation results in specific defects of boundary cell formation in the zebrafish hindbrain.