PUBLICATION
Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults
- Authors
- Horowitz, M., Milanese, C., Di Maio, R., Hu, X., Montero, L.M., Sanders, L.H., Tapias, V., Sepe, S., van Capellen, W.A., Burton, E.A., Greenamyre, T., and Mastroberardino, P.G.
- ID
- ZDB-PUB-110325-18
- Date
- 2011
- Source
- Antioxidants & redox signaling 15(4): 855-71 (Journal)
- Registered Authors
- Burton, Edward A., Sanders, Laurie
- Keywords
- none
- MeSH Terms
-
- Animals
- Astrocytes/cytology
- Brain Mapping/methods*
- Cells, Cultured
- Dopamine/physiology*
- Immunohistochemistry/methods*
- Neurons/cytology
- Neurons/physiology*
- Oxidation-Reduction
- Parkinson Disease/pathology
- Rats
- Reactive Oxygen Species/metabolism
- Single-Cell Analysis*
- Substantia Nigra/physiology*
- Zebrafish/physiology
- PubMed
- 21395478 Full text @ Antioxid. Redox Signal.
Citation
Horowitz, M., Milanese, C., Di Maio, R., Hu, X., Montero, L.M., Sanders, L.H., Tapias, V., Sepe, S., van Capellen, W.A., Burton, E.A., Greenamyre, T., and Mastroberardino, P.G. (2011) Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults. Antioxidants & redox signaling. 15(4):855-71.
Abstract
The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta (SNpc). These cells posses a distinct physiology intrinsically associated with elevated ROS production, and they selectively degenerate in Parkinson's disease (PD) under oxidative stress conditions. We developed a new imaging strategy to study redox variations in single cells that is sensitive enough to detect changes within the physiological range. We studied DA neurons' physiological redox response in biological systems of increasing complexity--from primary cultures to zebrafish larvae, to mammalian brains--and identified a redox response that is distinctive for SNpc DA neurons. We studied simultaneously, and in the same cells, redox state and signaling activation, and found that these phenomena are synchronized. While the new technique is of general interest, these findings provide insights into the biology of DA neurons in health and disease, and may have implications for therapeutic intervention.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping