ZFIN ID: ZDB-PUB-110325-18
Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults
Horowitz, M., Milanese, C., Di Maio, R., Hu, X., Montero, L.M., Sanders, L.H., Tapias, V., Sepe, S., van Capellen, W.A., Burton, E.A., Greenamyre, T., and Mastroberardino, P.G.
Date: 2011
Source: Antioxidants & redox signaling   15(4): 855-71 (Journal)
Registered Authors: Burton, Edward A., Sanders, Laurie
Keywords: none
MeSH Terms:
  • Animals
  • Astrocytes/cytology
  • Brain Mapping/methods*
  • Cells, Cultured
  • Dopamine/physiology*
  • Immunohistochemistry/methods*
  • Neurons/cytology
  • Neurons/physiology*
  • Oxidation-Reduction
  • Parkinson Disease/pathology
  • Rats
  • Reactive Oxygen Species/metabolism
  • Single-Cell Analysis*
  • Substantia Nigra/physiology*
  • Zebrafish/physiology
PubMed: 21395478 Full text @ Antioxid. Redox Signal.
The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta (SNpc). These cells posses a distinct physiology intrinsically associated with elevated ROS production, and they selectively degenerate in Parkinson's disease (PD) under oxidative stress conditions. We developed a new imaging strategy to study redox variations in single cells that is sensitive enough to detect changes within the physiological range. We studied DA neurons' physiological redox response in biological systems of increasing complexity--from primary cultures to zebrafish larvae, to mammalian brains--and identified a redox response that is distinctive for SNpc DA neurons. We studied simultaneously, and in the same cells, redox state and signaling activation, and found that these phenomena are synchronized. While the new technique is of general interest, these findings provide insights into the biology of DA neurons in health and disease, and may have implications for therapeutic intervention.